A New Subfamily of Short Bacterial Adenylate Kinases With the Mycobacterium tuberculosis Enzyme as a Model: A Predictive and Experimental Study

A New Subfamily of Short Bacterial Adenylate Kinases With the Mycobacterium tuberculosis Enzyme as a Model: A Predictive and Experimental Study

A New Subfamily of Short Bacterial Adenylate Kinases With the Mycobacterium tuberculosis Enzyme as a Model: A Predictive and Experimental Study

Abstract

The adk gene from Mycobacterium tuberculosis codes for an enzyme of 181 amino acids. A sequence comparison with 52 different forms ofadenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of “short” bacterial AKs. The recombinant protein, overexpressed in Escherichia coli, exhibits a low catalytic activity and an unexpectedly high thermal stability (Tm = 64.8 degrees C). Based on various spectroscopic data, on the known three-dimensional structure of the AK from E. coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt). Proteins 1999;36:238-248.

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