Analyzing Arthropods for the Presence of Bacteria

Analyzing Arthropods for the Presence of Bacteria

Analyzing Arthropods for the Presence of Bacteria

ABSTRACT

Bacteria within arthropods can be identified using culture-independent methods. This unit
describes protocols for surface sterilization of arthropods, DNA extraction of whole bodies
and tissues, touchdown PCR amplification using 16S rDNA general bacteria primers,
and profiling the bacterial community using denaturing gradient gel electrophoresis.

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