Fluorescence microscopy

Fluorescence microscopy is an important laboratory tool used in modern cell and molecular biology. Scientists use fluorescence microscopy to biochemically label and them detect molecules within the cell as part of basic biomedical research and for diagnostic purposes. Fluorescence Microscopy is the revised, updated and expanded second edition of a best-selling introductory text on the use of fluorescence microscopy in cell biology. The book covers the fundamental principles of fluorescence and their application to fluorescence microscopy. A detailed description of the fluorescence microscope is given too. Application to immunofluorescence, in situ hybridization, and photomicrography are presented in full, practical details. Troubleshooting guidance is provided, along with many practical hints, to guide the user through commonly encountered problems. Specific topics covered include: * Fundamentals of fluorescence * Components of fluorescence microscopes * Quantitative fluorescence microscopy * Green fluorescent proteins. * Fluorescence in situ hybridization (FISH) * Digitized video microscopy * Single and multiphoton microscopy * Fluorescence photomicroscopy Fluorescence Microscopy Second Edition will continue to be essential reading for those new to the field and will now provide a valuable update for more experienced users in the latest applications. Read more