Detection of a Nuclear Antigen 2 (EBNA2)Wariant Epstein-Barr Virus Strain in Two Siblings With Fatal Lymphoproliferative Disease

Detection of a Nuclear Antigen 2 (EBNA2)Wariant Epstein-Barr Virus Strain in Two Siblings With Fatal Lymphoproliferative Disease

Detection of a Nuclear Antigen 2 (EBNA2)Wariant Epstein-Barr Virus Strain in Two Siblings With Fatal Lymphoproliferative Disease

Abstract

An EBV type 1 variant strain was detected in two Turkish siblings (boy and girl), who both suffered and died from similar progressive Epstein-Barrvirus (EBV)-associated lymphoproliferative disease. Molecular characterisation of this EBV isolate revealed a 51bp-deletion and six nucleotide changes within the Epstein-Barr nuclear antigen 2 (EBNA2). Both isolates contained EBV type 2 sequences in the Epstein-Barr virus-encoded small RNAs (EBER), which are 40 kb proximal to EBNA2. Sequencing of the EBV isolates in a region of Epstein-Barr nuclear antigen 3 (EBNA3a), which is 40 kb distal to EBNA2, revealed the normal EBV type 1 sequence of laboratory strain B95-8. This EBV isolate may represent a distinct wild type EBV strain with altered biological properties. It is suggested that this EBNA2-variant strain may be responsible at least in part for the severe clinical course in both affected children.

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