Generation of High-Titer Defective HSV-1 Vectors

Generation of High-Titer Defective HSV-1 Vectors

Generation of High-Titer Defective HSV-1 Vectors

ABSTRACT

Basic Protocol 1 describes the generation of helper virus stocks. Preparation of recombinant
amplicon vector particles by transfection of amplicon and superinfection of helper
virus into cells, and harvesting of packaged particles, is delineated in Basic Protocol 2.
Thorough characterization of each amplicon viral vector stock involves measuring (1)
the helper virus plaque-forming units per ml (pfu/ml) on 2-2 cells and (2) the amplicon
stock infectious units per ml (iu/ml) on PC12 cells. The Support Protocols detail methods
for determining titers of helper virus by plaque assay, and of amplicon stocks by vector
assay.
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