Hierarchically Assembled Mesenchymal Stem Cell Spheroids Using Biomimicking Nanofilaments and Microstructured Scaffolds for Vascularized Adipose Tissue Engineering

Hierarchically Assembled Mesenchymal Stem Cell Spheroids Using Biomimicking Nanofilaments and Microstructured Scaffolds for Vascularized Adipose Tissue Engineering

Hierarchically Assembled Mesenchymal Stem Cell Spheroids Using Biomimicking Nanofilaments and Microstructured Scaffolds for Vascularized Adipose Tissue Engineering

Composite multicellular spheroids composed of mesenchymal stem cells
(MSCs) and synthetic biodegradable nanofilaments are fabricated.
Extracellular-matrix-mimicking nanofilaments, prepared from transverse
fragmentation of semicrystalline poly(L-lactic acid) nanofibers and
subsequent surface modification with cell adhesive peptides, are used to form
composite multicellular spheroids with MSCs by cellular self-assembly. The
size of the composite spheroids could be readily controlled with the integrated
amount of the nanofilaments. The composite spheroids show enhanced
adipogenic potential compared to homotypic spheroids. The resultant
spheroids are used as building blocks for 3D biohybrid construction with the
assistance of a microstructured scaffold fabricated by a direct polymer melt
deposition process. An angiogenic growth factor, basic fibroblast growth
factor, is also locally delivered in a sustained fashion from the heparinized
scaffold surface for facile neovascularization of adipogenic tissue. The
produced multiscaled and multifunctional hybrid MSC construct enable the
successful formation of vascularized adipose tissue in vivo.

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