Immunological Analysis of Apolipophorin-l I I in the Haemolymph, Ovaries, and Testes of the Fall Webworm, Hyphantria cunea (Drury)

Immunological Analysis of Apolipophorin-l I I in the Haemolymph, Ovaries, and Testes of the Fall Webworm, Hyphantria cunea (Drury)

Immunological Analysis of Apolipophorin-l I I in the Haemolymph, Ovaries, and Testes of the Fall Webworm, Hyphantria cunea (Drury)

Abstract

Apolipophorin-Ill (apolp-ill) was purified from the haemolymph of adult Hyphantrid tuned (Drury) by KBr density gradient ultracentrifugation, gel filtra-tion (Sephadex G-100) and ion exchange chromatography (CM-52), and its characteristics, molecular weight, tissue distribution, and sites of synthesis were examined. Molecular weight of apolp-Ill was estimated to be 18 kDa. Byelec-trophoretic analysis on gels of male and female haemolyrnph from di-verse developmental stages, apolp-Ill was shown to be present in all stages. Western blotting was carried out to show that purified free apolp-Ill is iden-tical to apolp-Ill associated with adult lipophorin. lrnrnunological analysis also showed that apolp-Ill is present in the ovary and the testis and in the case of testis, apolp-Ill is heavily accumulated in the cyst. ApoLp-Ill is synthesized in larval and adult Cat body but not in adult testis. Autoradiography following incubation of [‘4C]apoLp-lII with testis showed that apolp-Ill was taken up
into testis.

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