Isolation of Herpes Simplex Virus DNA from Virus Particles and Infected Cells by Electrophoresis in Polyacrylamide Gels

Isolation of Herpes Simplex Virus DNA from Virus Particles and Infected Cells by Electrophoresis in Polyacrylamide Gels

Isolation of Herpes Simplex Virus DNA from Virus Particles and Infected Cells by Electrophoresis in Polyacrylamide Gels

SUMMARY :

The DNA molecules synthesized in herpes simplex virus (HSV) infected cells
were analysed by electrophoresis in polyacrylamide gels. It was found that part of
the virus DNA, isolated from infected nuclei, migrated into the gels in a manner
similar to the virus DNA molecules isolated from purified herpes simplex virus
particles. Most of the virus DNA molecules, labelled in vivo for 60 min with
[3H]-thymidine as well as labelled cellular DNA molecules, were retained at the
top of the gel during electrophoresis. The amount of HSV-DNA which migrated
into the gel gradually increased when the labelled cells were reincubated after
removal of the isotope (chase). Most of the DNA molecules, which were retained
at the top of the gel, had the density of virus DNA but differed in their sedimenta-
tion behaviour in sucrose gradients from the intact DNA molecules isolated from
purified virus particles. The presence of replicating as well as mature virus DNA
molecules in infected nuclei is discussed.

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