Laboratory Maintenance of Methicillin‐Resistant Staphylococcus aureus (MRSA)

Laboratory Maintenance of Methicillin‐Resistant Staphylococcus aureus (MRSA)

Laboratory Maintenance of Methicillin‐Resistant Staphylococcus aureus (MRSA)

ABSTRACT

Staphylococcus aureus is an important bacterial pathogen in the hospital and community
settings, especially Staphylococcus aureus clones that exhibit methicillin-resistance
(MRSA).Many strains of S. aureus are utilized in the laboratory, underscoring the genetic
differences inherent in clinical isolates. S. aureus grows quickly at 37◦C with aeration
in rich media (e.g., BHI) and exhibits a preference for glycolytic carbon sources. Furthermore,
S. aureus has a gold pigmentation, exhibits β-hemolysis, and is catalase and
coagulase positive. The four basic laboratory protocols presented in this unit describe
how to culture S. aureus on liquid and solid media, how to identify S. aureus strains
as methicillin resistant, and how to generate a freezer stock of S. aureus for long-term
storage.
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