Measuring Intravascular Migration of Mouse Ly6Clow Monocytes In Vivo Using Intravital Microscopy

Measuring Intravascular Migration of Mouse Ly6Clow Monocytes In Vivo Using Intravital Microscopy

Measuring Intravascular Migration of Mouse Ly6Clow Monocytes In Vivo Using Intravital Microscopy

ABSTRACT

This unit describes methods for intravital imaging of monocytes in the vasculature of the
dermis and the mesentery in vivo using fluorescent reporter mice, fluorescent dyes, and
antibodies. Cx3cr1gfp/gfp (or +), Rag2−/−, Il2rg−/− mice expressing eGFP at the locus of
the Cx3cr1 gene, on the Rag2−/− Il2rg−/− C57Bl/6 background, are used. Although aimed
at specifically tracking Ly6Clow monocytes, these protocols could readily be adapted to
investigate the interaction of other blood leukocytes with the vascular endothelium by
use of other fluorescent reporter mice and fluorescently labeled antibodies.

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