Mouse Model for Pyelonephritis

Mouse Model for Pyelonephritis

Mouse Model for Pyelonephritis

ABSTRACT

This unit describes a simple way to induce pyelonephritis in female mice using
uropathogenic Escherichia coli (UPEC). Methods for culturing and preparing working
stocks of UPEC are provided. Protocols to measure the bacterial load in the murine
kidney following the establishment of pyelonephritis by determining the bacterial colony
forming units (CFU) are also included. This assay can be performed with kidney homogenates
if the bacterial load is the sole read-out or if cellular intactness is not required
for subsequent assays, or from kidney single cell suspensions prepared by enzymatic
digestion. A support protocol describes methods for isolating cells for flow cytometry,
ex vivo culture, and other assays.

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