Myogenic Induction of Aligned Mesenchymal Stem Cell Sheets by Culture on Thermally Responsive Electrospun Nanofibers*

Myogenic Induction of Aligned Mesenchymal Stem Cell Sheets by Culture on Thermally Responsive Electrospun Nanofibers*

Myogenic Induction of Aligned Mesenchymal Stem Cell Sheets by Culture on Thermally Responsive Electrospun Nanofibers*

Exploration of artificial, nanometer-scale features to mimic
natural matrices has revealed a powerful influence of nanotopography over cellular behavior. In this study, aligned nanofibers composed of thermally responsive hydroxybutyl
chitosan were electrospun to create a robust scaffold for the
production of aligned cell sheets. With a median fiber diameter of 436 nm, fiber alignment created repetitively spaced
nanometer-scale topographical features on the scaffold surface. When cultured on these surfaces, human mesenchymal
stem cells (hMSC) showed alignment and elongation in both
cell body and nucleus. In addition to morphological changes,
topographical features induced expression of genes indicative
of myogenic induction of hMSC cultured in proliferative, nondifferentiating medium. Thermal reversibility of the fibers allows for the dissolution of the polymer from the cell/scaffold
construct without disruption of cytoskeletal structure and
cell–cell interactions. For cell-based regenerative medicine
applications, thermally reversible nanofibers can thus produce
polymer-free cell layers engineered with exposure to nanotopographical cues.

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