Poliovirus: Generation and Characterization of Mutants

Poliovirus: Generation and Characterization of Mutants

Poliovirus: Generation and Characterization of Mutants

ABSTRACT

Poliovirus (PV) is the prototypical picornavirus. It is a non-enveloped RNA virus with a
small (∼7.5-kb) genome of positive polarity. cDNA clones of several strains are available,
and infectious virus can be produced by the transfection of in vitro–transcribed
viral genomes into an appropriate host cell. The ease of genetic studies in poliovirus is
a primary reason that it has long served as a model to study RNA virus biology, pathogenesis,
and evolution. Protocols for the generation and characterization of PV mutants
are presented.
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