Poliovirus: Generation, Quantification, Propagation, Purification, and Storage

Poliovirus: Generation, Quantification, Propagation, Purification, and Storage

Poliovirus: Generation, Quantification, Propagation, Purification, and Storage

ABSTRACT

Poliovirus (PV) is the prototypical picornavirus. It is a non-enveloped RNA virus with
a small (∼7.5-kb) genome of positive polarity. It has long served as a model to study
RNA virus biology, pathogenesis, and evolution. cDNA clones of several strains are
available, and infectious virus can be produced by the transfection of in vitro transcribed
viral genomes into an appropriate host cell. PV infects many human and non-human
primate cell lines including HeLa and HeLa S3 cells, and can grow to high titer in
culture. Protocols for the production, propagation, quantification, and purification of PV
are presented.

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