Production of Recombinant Bacterial Endoglucanase as a Co-Product with Ethanol During Fermentation Using Derivatives of Escherichia coli KO11

Production of Recombinant Bacterial Endoglucanase as a Co-Product with Ethanol During Fermentation Using Derivatives of Escherichia coli KO11

Production of Recombinant Bacterial Endoglucanase as a Co-Product with Ethanol During Fermentation Using Derivatives of Escherichia coli KO11

Abstract

This study demonstrates a new approach to reduce the amount of fungal cellulase required for the conversion of cellulose into ethanol. Escherichiacoli KO11, a biocatalyst developed for the fermentation of hemicellulose syrups, was used to produce recombinant endoglucanase as a co-productwith ethanol. Seven different bacterial genes were expressed from plasmids in KO11. All produced cell-associated endoglucanase activity.KO11(pLOI1620) containing Erwinia chrysanthemi celZ (EGZ) produced the highest activity, 3,200 IU endoglucanase/L fermentation broth (assayed at pH 5.2 and 35 degrees C). Recombinant EGZ was solubilized from harvested cells by treatment with dilute sodium dodecyl sulfate (12.5 mg/ml, 10 min, 50 degrees C) and tested in fermentation experiments with commercial fungal cellulase (5 filter paper units/g cellulose) and purified cellulose (100 g/L). Using Klebsiella oxytoca P2 as the biocatalyst, fermentations supplemented with EGZ as a detergent-lysate of KO11(pLOI1620) produced 14%-24% more ethanol than control fermentations supplemented with a detergent-lysate of KO11(pUC18). These results demonstrate thatrecombinant bacterial endoglucanase can function with fungal cellulase to increase ethanol yield during the simultaneous saccharification andfermentation of cellulose.

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