Quantification of microRNA Expression with Next-Generation Sequencing

Quantification of microRNA Expression with Next-Generation Sequencing

Quantification of microRNA Expression with Next-Generation Sequencing

ABSTRACT

Rapid advancement of next-generation sequencing technologies has made it possible
to study expression profiles of microRNAs (miRNAs) comprehensively and efficiently.
Multiplexing miRNA libraries by barcoding can significantly reduce sequencing cost per
sample without compromising library quality. This unit provides a step-by-step protocol
for isolating miRNAs and constructing multiplexed miRNA libraries. Also described is a
custom computational pipeline for analyzing the multiplexed miRNA library sequencing
reads generated by Illumina-based technology.
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