Quick Bacterial Microwave Fixation Technique for Scanning Electron Microscopy

Quick Bacterial Microwave Fixation Technique for Scanning Electron Microscopy

Quick Bacterial Microwave Fixation Technique for Scanning Electron Microscopy

The times required for routine fixation of bacteria for
scanning electron microscopy (SEM) requires a lengthy
process (Amako and Umeda, 1977; Galli et al., 1989;
Krogstad et al., 1980; Wanner et al., 1989). Microwave
irradiation is an excellent method for preservation of
cell structure for electron microscopy and significantly
reduces processing times (Giberson and Demaree, 1995,
1999; Giberson et al., 1995, 1997; Login and Dvorak,
1994). The purpose of this study was to introduce and
compare the routine fixation process with a new protocol utilizing microwave irradiation. The time necessary
to prepare samples for SEM can take up to 4 hours.
Using a new microwave technique, the time can be
reduced to approximately 1 hour.

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