RNA Isolation from Mammalian Samples

RNA Isolation from Mammalian Samples

RNA Isolation from Mammalian Samples

ABSTRACT

RNAcan be extracted from cultured cells, peripheral blood, bone marrow, plasma, serum,
body fluids, and fresh or frozen tissues. RNA can also be extracted from formalin-fixed
paraffin-embedded (FFPE) tissues. Methods for RNA extraction can be divided into
three groups: phenol/chloroform extraction, silica spin-column absorption, and isopycnic
gradient centrifugation. Two differentRNAisolation procedures are described in this unit.
The first basic protocol describes a one-step isolation involving monophasic lysis with
guanidine isothiocyanate and phenol followed by chloroform extraction. The second
basic protocol describes a silica-column separation method for RNA isolation.
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