Sequencing of a 17-6 kb Segment on the Right Arm of Yeast Chromosome VII Reveals 12 ORFs, Including CCT, ADE3 and TR-I Genes, Homologues of the Yeast PMT and EFIG Genes, of the Human and Bacterial Electron-Transferring Flavoproteins (P-chain) and of the Escherichia coli Phosphoserine Phosphohydrolase, and Five New ORFs

Sequencing of a 17-6 kb Segment on the Right Arm of Yeast Chromosome VII Reveals 12 ORFs, Including CCT, ADE3 and TR-I Genes, Homologues of the Yeast PMT and EFIG Genes, of the Human and Bacterial Electron-Transferring Flavoproteins (P-chain) and of the Escherichia coli Phosphoserine Phosphohydrolase, and Five New ORFs

Sequencing of a 17-6 kb Segment on the Right Arm of Yeast Chromosome VII Reveals 12 ORFs, Including CCT, ADE3 and TR-I Genes, Homologues of the Yeast PMT and EFIG Genes, of the Human and Bacterial Electron-Transferring Flavoproteins (P-chain) and of the Escherichia coli Phosphoserine Phosphohydrolase, and Five New ORFs

Abstract

A 17.6 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced
and analysed. The sequence contains twelve open reading frames (ORFs) longer than 100 amino acids. Three genes
had already been cloned and sequenced: CCT, ADE3 and TR-I. Two ORFs are similar to other yeast genes: G7722
with the YAL023 (PMT2) and PMTl genes, encoding two integral membrane proteins, and G7727 with the first half
of the genes encoding elongation factors ly, TEF3 and TEF4. Two other ORFs, G7742 and G7744, are most
probably yeast orthologues of the human and Paracoccus denitrificans electron-transferring flavoproteins (p chain)
and of the Escherichia coli phosphoserine phosphohydrolase. The five remaining identified ORFs do not show
detectable homology with other protein sequences deposited in data banks. The sequence has been deposited in the
EMBL data library under Accession Number 249133.

read online

Post Comment