Vitellogenins and Vitellins in the Bean Bug, Ripfortus clavatus (Hemiptera: Al ydidae): Purification, Immunological Identification, and Induction by Juvenile Hormone

Vitellogenins and Vitellins in the Bean Bug, Ripfortus clavatus (Hemiptera: Al ydidae): Purification, Immunological Identification, and Induction by Juvenile Hormone

Vitellogenins and Vitellins in the Bean Bug, Ripfortus clavatus (Hemiptera: Al ydidae): Purification, Immunological Identification, and Induction by Juvenile Hormone

Abstract

Vitellogenins (Vgs) and vitellins (Vns) were purified from reproductive female adult hemolymph (HL) and newly laid eggs of the bean bug, Riptortus clavatus. They vvere separated into two (Vg-I and 2) and three (Vn-1 ‘, Vn-I, and Vn- 2) sub-components, respectively, by ion exchange chromatography. Fused rocket immunoelectrophoresis using anti-(Vn-1) serum showed that they are distinguished clearly into two immunologically distinct groups, i.e., Vg-l/ Vn-l/Vn-1 ’ and Vg-2/Vn-2. SDS-PAGE analysis showed Vn-1 (Vn-1 ‘) and
Vn-2 have the same and/or smaller subunit component polypeptides as Vg-
1 and Vg-2, respectively. Vn-1 ’ has some lower molecular weight peptides
than Vn-1 . Quantitative rocket immunoelectrophoretic analysis showed that
Vg-1 and Vn-I (plus V-I ’) were detected first on day 3 in the HL and day 4
in the ovary, respectively, after adult emergence, and increased by day 10
in non-diapause female adults. Vg-1 synthesis is induced in diapause ie-
male adults in 1 day after the treatment with juvenile hormone (JH) and JH
analog (JHA) or in a few days after transfer from short day to long day
conditions.

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